I'm a researcher by trade and so I have been reading and reading so here is a post from Dr. H
A large majority of patients with myeloproliferative neoplasms (MPNs) have a mutation in the JAK2 kinase, JAK2V617F, which creates disease by increasing CDC25A translation. Another line of research shows you can minimize the actual CDC25A disease process through indole-3-carbinol.
Gautier et al., The cell cycle regulator CDC25A is a target for JAK2V617F oncogene. Blood. 2012 Feb 2;119(5):1190-9.
The authors show that JAK2V617F increases CDC25A translation. This is effected by a
novel signaling pathway in which JAK2V617F decreases phosphorylation of elongation factor
eIF2_, thereby retaining or prolonging its activity, resulting in increased CDC25A translation.
Importantly, pharmacologic inhibition of JAK2V617F-mediated eIF2_ dephosphorylation
decreased proliferation of primary MPN cells. Furthermore, growth of EPOindependent
erythroid colonies (so-called endogenous erythroid colonies [EECs]), a pathognomonic
hallmark of polycythemia vera,6 was selectively and significantly inhibited by addition
of a pan-CDC25 inhibitor. These data demonstrate that mutant JAK2V617F actively influences cell-cycle regulation, thereby promoting cell proliferation. Notably, the study establishes CDC25A as a novel JAK2V617F target and suggests that its inhibition may impede growth of the MPN clone.
The natural compound indole-3-carbinol (I3C; found in vegetables of the genus Brassica) is a promising cancer prevention or therapy agent. The cell division cycle 25A (Cdc25A) phosphatase is overexpressed in a variety of human cancers and other diseases. In the present study, I3C induced degradation of Cdc25A, arrest of the G1 cell cycle, and inhibition of the growth of cancer cells. Study showed that the Ser124 site of Cdc25A, which is related to cyclin-dependent kinase 2, is required for I3C-induced degradation of Cdc25A in cancer cells, and that interruption of the ATM-Chk2 pathway suppressed I3C-induced destruction of Cdc25A.
Cancer Prev Res (Phila). 2010 Jul;3(7):818-28. Epub 2010 Jun 29.
A novel mechanism of indole-3-carbinol effects on breast carcinogenesis involves induction of Cdc25A degradation.
Wu Y, Feng X, Jin Y, Wu Z, Hankey W, Paisie C, Li L, Liu F, Barsky SH, Zhang W, Ganju R, Zou X.
Glucosinolate Content of Selected Cruciferous Vegetables (90)
½ cup (25 g)
½ cup, chopped (28 g)
½ cup (44 g)
1 tablespoon (15 g)
1 cup, chopped (67 g)
1 cup, chopped (34 g)
½ cup, cubes (65 g)
½ cup, chopped (45 g)
½ cup, chopped (45 g)
½ cup, chopped (44 g)
½ cup, chopped (67 g)
Bok choi (pak choi)
½ cup, chopped (35 g)
½ cup, chopped (50 g)